The man's body was recovered and pieces of tissue were studied for their C content by accelerator mass spectroscopy.The best estimate from this dating technique says the man lived between 33 BC. From the ratio, the time since the formation of the rock can be calculated. Along with hydrogen, nitrogen, oxygen, phosphorus, and sulfur, carbon is a building block of biochemical molecules ranging from fats, proteins, and carbohydrates to active substances such as hormones.
Levels of carbon-14 become difficult to measure and compare after about 50,000 years (between 8 and 9 half lives; where 1% of the original carbon-14 would remain undecayed).
The question should be whether or not carbon-14 can be used to date any artifacts at all? There are a few categories of artifacts that can be dated using carbon-14; however, they cannot be more 50,000 years old.
Carbon-14 cannot be used to date biological artifacts of organisms that did not get their carbon dioxide from the air.
This rules out carbon dating for most aquatic organisms, because they often obtain at least some of their carbon from dissolved carbonate rock.
Signals of this kind are often used by chemists studying natural environments.
A hydrocarbon found in beach sediments, for example, might derive from an oil spill or from waxes produced by plants.The pathway from the plant to the molecule may have been indirect or lengthy, involving multiple physical, chemical, and biological processes.Levels of C can represent either mixtures of modern and dead carbon or carbon that was fixed from the atmosphere less than 50,000 years ago.Ninety-nine percent of these also contain six neutrons.The 6 proton 6 neutron atoms are said to have a mass of 12 and are referred to as "carbon-12." The nuclei of the remaining one percent of carbon atoms contain not six but either seven or eight neutrons in addition to the standard six protons.The age of the carbon in the rock is different from that of the carbon in the air and makes carbon dating data for those organisms inaccurate under the assumptions normally used for carbon dating.